Plant Transcription Factor Database
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT2G22770.1
Common NameBHLH20, EN27, NAI1, T30L20.3
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 320aa    MW: 35697.5 Da    PI: 7.4114
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT2G22770.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
          HLH   4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                   h  +Er+RR+++N+++  L  llP +     kK +Ka++Le A++++k+Lq
                  5889***********************.....9******************9 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5088815.745128177IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474591.06E-15129192IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:, basic helix-loop-helix (bHLH) domain
PfamPF000101.9E-10132178IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000834.71E-12133182No hitNo description
SMARTSM003532.8E-14134183IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0007029Biological Processendoplasmic reticulum organization
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000037anatomyshoot apex
PO:0000293anatomyguard cell
PO:0009025anatomyvascular leaf
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 320 aa     Download sequence    Send to blast
Nucleic Localization Signal ? help Back to Top
No. Start End Sequence
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT2G22770-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems, and flowers. {ECO:0000269|PubMed:12679534}.
Functional Description ? help Back to Top
Source Description
TAIRregulates the development of ER bodies. also involves in response to the endophytic fungus Piriformospora indica.
UniProtTranscription activator that regulates the expression of at least NAI2, PYK10 and PBP1. Required for and mediates the formation of endoplasmic reticulum bodies (ER bodies). Involved in the symbiotic interactions with the endophytes of the Sebacinaceae fungus family, such as Piriformospora indica and Sebacina. {ECO:0000269|PubMed:12581307, ECO:0000269|PubMed:15155889, ECO:0000269|PubMed:15919674, ECO:0000269|PubMed:18248598, ECO:0000269|PubMed:18467340, ECO:0000269|PubMed:18780803}.
Function -- GeneRIF ? help Back to Top
  1. role in the formation of endoplasmic reticulum bodies
    [PMID: 15155889]
Cis-element ? help Back to Top
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G39310(A), AT2G39330(A), AT3G09260(A), AT3G15950(A), AT3G16420(A), AT3G16430(A)
Interaction ? help Back to Top
Source Intact With
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT2G22770
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF4885640.0AF488564.1 Arabidopsis thaliana clone bHLH020 putative bHLH transcription factor mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_850031.20.0transcription factor NAI1
SwissprotQ8S3F10.0BH020_ARATH; Transcription factor NAI1
TrEMBLD7LE350.0D7LE35_ARALL; Putative uncharacterized protein
STRINGAT2G22770.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP6561573
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  2. Ma L, et al.
    Genomic evidence for COP1 as a repressor of light-regulated gene expression and development in Arabidopsis.
    Plant Cell, 2002. 14(10): p. 2383-98
  3. Matsushima R,Kondo M,Nishimura M,Hara-Nishimura I
    A novel ER-derived compartment, the ER body, selectively accumulates a beta-glucosidase with an ER-retention signal in Arabidopsis.
    Plant J., 2003. 33(3): p. 493-502
  4. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
  5. Hudson ME,Lisch DR,Quail PH
    The FHY3 and FAR1 genes encode transposase-related proteins involved in regulation of gene expression by the phytochrome A-signaling pathway.
    Plant J., 2003. 34(4): p. 453-71
  6. Matsushima R, et al.
    The ER body, a novel endoplasmic reticulum-derived structure in Arabidopsis.
    Plant Cell Physiol., 2003. 44(7): p. 661-6
  7. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
  8. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
  9. Matsushima R,Fukao Y,Nishimura M,Hara-Nishimura I
    NAI1 gene encodes a basic-helix-loop-helix-type putative transcription factor that regulates the formation of an endoplasmic reticulum-derived structure, the ER body.
    Plant Cell, 2004. 16(6): p. 1536-49
  10. Kiba T, et al.
    Combinatorial microarray analysis revealing arabidopsis genes implicated in cytokinin responses through the His->Asp Phosphorelay circuitry.
    Plant Cell Physiol., 2005. 46(2): p. 339-55
  11. Nagano AJ,Matsushima R,Hara-Nishimura I
    Activation of an ER-body-localized beta-glucosidase via a cytosolic binding partner in damaged tissues of Arabidopsis thaliana.
    Plant Cell Physiol., 2005. 46(7): p. 1140-8
  12. Devoto A, et al.
    Expression profiling reveals COI1 to be a key regulator of genes involved in wound- and methyl jasmonate-induced secondary metabolism, defence, and hormone interactions.
    Plant Mol. Biol., 2005. 58(4): p. 497-513
  13. Pischke MS,Huttlin EL,Hegeman AD,Sussman MR
    A transcriptome-based characterization of habituation in plant tissue culture.
    Plant Physiol., 2006. 140(4): p. 1255-78
  14. Mandaokar A, et al.
    Transcriptional regulators of stamen development in Arabidopsis identified by transcriptional profiling.
    Plant J., 2006. 46(6): p. 984-1008
  15. Feller A,Hernandez JM,Grotewold E
    An ACT-like domain participates in the dimerization of several plant basic-helix-loop-helix transcription factors.
    J. Biol. Chem., 2006. 281(39): p. 28964-74
  16. Lee DJ, et al.
    Genome-wide expression profiling of ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) overexpression in cytokinin response.
    Mol. Genet. Genomics, 2007. 277(2): p. 115-37
  17. Sherameti I, et al.
    PYK10, a beta-glucosidase located in the endoplasmatic reticulum, is crucial for the beneficial interaction between Arabidopsis thaliana and the endophytic fungus Piriformospora indica.
    Plant J., 2008. 54(3): p. 428-39
  18. Nagano AJ,Fukao Y,Fujiwara M,Nishimura M,Hara-Nishimura I
    Antagonistic jacalin-related lectins regulate the size of ER body-type beta-glucosidase complexes in Arabidopsis thaliana.
    Plant Cell Physiol., 2008. 49(6): p. 969-80
  19. Yamada K,Nagano AJ,Nishina M,Hara-Nishimura I,Nishimura M
    NAI2 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana.
    Plant Cell, 2008. 20(9): p. 2529-40
  20. Tominaga-Wada R,Iwata M,Nukumizu Y,Wada T
    Analysis of IIId, IIIe and IVa group basic-helix-loop-helix proteins expressed in Arabidopsis root epidermis.
    Plant Sci., 2011. 181(4): p. 471-8
  21. Yamada K,Hara-Nishimura I,Nishimura M
    Unique defense strategy by the endoplasmic reticulum body in plants.
    Plant Cell Physiol., 2011. 52(12): p. 2039-49
  22. Nakano RT, et al.
    ERMO3/MVP1/GOLD36 is involved in a cell type-specific mechanism for maintaining ER morphology in Arabidopsis thaliana.
    PLoS ONE, 2012. 7(11): p. e49103
  23. Yamada K,Nagano AJ,Nishina M,Hara-Nishimura I,Nishimura M
    Identification of two novel endoplasmic reticulum body-specific integral membrane proteins.
    Plant Physiol., 2013. 161(1): p. 108-20
  24. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
  25. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73